prism 7000 sequence detection system (Thermo Fisher)
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prism 7000 sequence detection system
Prism 7000 Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/7000+sequence+detection+system/pm40580687-99-42-41
Average 90 stars, based on 1 article reviews
Prism 7000 Sequence Detection System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/7000+sequence+detection+system/pm40580687-99-42-41
Average 90 stars, based on 1 article reviews
prism 7000 sequence detection system - by Bioz Stars,
2026-09
90/100 stars
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Polymerase Chain Reaction:Article Title: Modafinil exerts anti-inflammatory and anti-fibrotic effects by upregulating adenosine A 2A and A 2B receptors Article Snippet: Quantitative polymerase chain reaction (PCR) RNA isolation was performed using Trizol reagent (Invitrogen, Carlsbad, CA, USA), and RNA was then reverse-transcribed using a high-capacity complementary DNA archive kit (Applied Biosystems, Foster City, CA, USA). .. PCR was performed on an ABI Article Title: A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay Article Snippet: The thermocycler conditions were 95 °C for 5 min, followed by 25–35 cycles of 94 °C for 30 s, 52–60 °C for 60 s, and 72 °C for 90 s, with a final extension of 72 °C for 5 min. Ten-microliter aliquots from each RT-PCR sample were analyzed by electrophoresis on 2% agarose gels. .. RT-qPCR was performed with the ABI Article Title: A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay Article Snippet: The thermocycler conditions were 95 ◦C for 5 min, followed by 25–35 cycles of 94 ◦C for 30 s, 52–60 ◦C for 60 s, and 72 ◦C for 90 s, with a final extension of 72 ◦C for 5 min. Ten-microliter aliquots from each RT-PCR sample were analyzed by electrophoresis on 2% agarose gels. .. RT-qPCR was performed with the ABI Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions Article Snippet: Total RNA was extracted using RNeasy Plus Micro kit (Qiagen) according to the manufacturer’s protocol followed by cDNA synthesis using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. Real-time PCR was performed on an ABI Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions. Article Snippet: Objective: Dynorphin, an endogenous opioid peptide predominantly expressed in the central nervous system and involved in stress response, pain, and addiction, has intrigued researchers due to its expression in pancreatic b-cells.. In this study, we aimed to characterize dynorphin expression in mouse and human islets and explore the mechanisms regulating its expression.. Methods: We used primary mouse and human islets with unbiased published datasets to examine how glucose and other nutrients regulate dynorphin expression and secretion in islets. Article Title: Serum amyloid P (PTX2) attenuates hepatic fibrosis in mice by inhibiting the activation of fibrocytes and HSCs Article Snippet: RNA was isolated from livers or isolated HSCs using TRIzol (Invitrogen) followed by an RNeasy kit (Qiagen). .. Quantitative reverse transcription polymerase chain reaction was performed using an ABI Sequencing:Article Title: Modafinil exerts anti-inflammatory and anti-fibrotic effects by upregulating adenosine A 2A and A 2B receptors Article Snippet: Quantitative polymerase chain reaction (PCR) RNA isolation was performed using Trizol reagent (Invitrogen, Carlsbad, CA, USA), and RNA was then reverse-transcribed using a high-capacity complementary DNA archive kit (Applied Biosystems, Foster City, CA, USA). .. PCR was performed on an ABI Article Title: A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay Article Snippet: The thermocycler conditions were 95 °C for 5 min, followed by 25–35 cycles of 94 °C for 30 s, 52–60 °C for 60 s, and 72 °C for 90 s, with a final extension of 72 °C for 5 min. Ten-microliter aliquots from each RT-PCR sample were analyzed by electrophoresis on 2% agarose gels. .. RT-qPCR was performed with the ABI Article Title: MCP5, a methyl-accepting chemotaxis protein regulated by both the Hk1-Rrp1 and Rrp2-RpoN-RpoS pathways, is required for the immune evasion of Borrelia burgdorferi Article Snippet: .. The samples were assayed in triplicate using the ABI Article Title: A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay Article Snippet: The thermocycler conditions were 95 ◦C for 5 min, followed by 25–35 cycles of 94 ◦C for 30 s, 52–60 ◦C for 60 s, and 72 ◦C for 90 s, with a final extension of 72 ◦C for 5 min. Ten-microliter aliquots from each RT-PCR sample were analyzed by electrophoresis on 2% agarose gels. .. RT-qPCR was performed with the ABI Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions Article Snippet: Total RNA was extracted using RNeasy Plus Micro kit (Qiagen) according to the manufacturer’s protocol followed by cDNA synthesis using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. Real-time PCR was performed on an ABI Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions. Article Snippet: Objective: Dynorphin, an endogenous opioid peptide predominantly expressed in the central nervous system and involved in stress response, pain, and addiction, has intrigued researchers due to its expression in pancreatic b-cells.. In this study, we aimed to characterize dynorphin expression in mouse and human islets and explore the mechanisms regulating its expression.. Methods: We used primary mouse and human islets with unbiased published datasets to examine how glucose and other nutrients regulate dynorphin expression and secretion in islets. Article Title: Inhibitory Effect of TCF7L2 on Pancreatic β-Cell Dedifferentiation via ERK/MAPK Signaling Pathway in Diabetes Article Snippet: Total RNA was extracted from the cell lines using TRIzol Reagent (Thermo Fisher Scientific, Inc.) according to the manufacturer’s instructions. .. Reverse transcription was performed using a Reverse Transcription Kit (Thermo Fisher Scientific, Inc.). qPCR was performed in triplicate using the SYBR Green Master Mix (Takara Bio, Inc., Otsu, Japan) on an ABI Article Title: Serum amyloid P (PTX2) attenuates hepatic fibrosis in mice by inhibiting the activation of fibrocytes and HSCs Article Snippet: RNA was isolated from livers or isolated HSCs using TRIzol (Invitrogen) followed by an RNeasy kit (Qiagen). .. Quantitative reverse transcription polymerase chain reaction was performed using an ABI SYBR Green Assay:Article Title: Modafinil exerts anti-inflammatory and anti-fibrotic effects by upregulating adenosine A 2A and A 2B receptors Article Snippet: Quantitative polymerase chain reaction (PCR) RNA isolation was performed using Trizol reagent (Invitrogen, Carlsbad, CA, USA), and RNA was then reverse-transcribed using a high-capacity complementary DNA archive kit (Applied Biosystems, Foster City, CA, USA). .. PCR was performed on an ABI Article Title: A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay Article Snippet: The thermocycler conditions were 95 °C for 5 min, followed by 25–35 cycles of 94 °C for 30 s, 52–60 °C for 60 s, and 72 °C for 90 s, with a final extension of 72 °C for 5 min. Ten-microliter aliquots from each RT-PCR sample were analyzed by electrophoresis on 2% agarose gels. .. RT-qPCR was performed with the ABI Article Title: MCP5, a methyl-accepting chemotaxis protein regulated by both the Hk1-Rrp1 and Rrp2-RpoN-RpoS pathways, is required for the immune evasion of Borrelia burgdorferi Article Snippet: .. The samples were assayed in triplicate using the ABI Article Title: A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay Article Snippet: The thermocycler conditions were 95 ◦C for 5 min, followed by 25–35 cycles of 94 ◦C for 30 s, 52–60 ◦C for 60 s, and 72 ◦C for 90 s, with a final extension of 72 ◦C for 5 min. Ten-microliter aliquots from each RT-PCR sample were analyzed by electrophoresis on 2% agarose gels. .. RT-qPCR was performed with the ABI Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions Article Snippet: Total RNA was extracted using RNeasy Plus Micro kit (Qiagen) according to the manufacturer’s protocol followed by cDNA synthesis using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. Real-time PCR was performed on an ABI Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions. Article Snippet: Objective: Dynorphin, an endogenous opioid peptide predominantly expressed in the central nervous system and involved in stress response, pain, and addiction, has intrigued researchers due to its expression in pancreatic b-cells.. In this study, we aimed to characterize dynorphin expression in mouse and human islets and explore the mechanisms regulating its expression.. Methods: We used primary mouse and human islets with unbiased published datasets to examine how glucose and other nutrients regulate dynorphin expression and secretion in islets. Article Title: Inhibitory Effect of TCF7L2 on Pancreatic β-Cell Dedifferentiation via ERK/MAPK Signaling Pathway in Diabetes Article Snippet: Total RNA was extracted from the cell lines using TRIzol Reagent (Thermo Fisher Scientific, Inc.) according to the manufacturer’s instructions. .. Reverse transcription was performed using a Reverse Transcription Kit (Thermo Fisher Scientific, Inc.). qPCR was performed in triplicate using the SYBR Green Master Mix (Takara Bio, Inc., Otsu, Japan) on an ABI Quantitative RT-PCR:Article Title: A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay Article Snippet: The thermocycler conditions were 95 °C for 5 min, followed by 25–35 cycles of 94 °C for 30 s, 52–60 °C for 60 s, and 72 °C for 90 s, with a final extension of 72 °C for 5 min. Ten-microliter aliquots from each RT-PCR sample were analyzed by electrophoresis on 2% agarose gels. .. RT-qPCR was performed with the ABI Article Title: A Comparative Overview of the Role of Human Ribonucleases in Nonsense-Mediated mRNA Decay Article Snippet: The thermocycler conditions were 95 ◦C for 5 min, followed by 25–35 cycles of 94 ◦C for 30 s, 52–60 ◦C for 60 s, and 72 ◦C for 90 s, with a final extension of 72 ◦C for 5 min. Ten-microliter aliquots from each RT-PCR sample were analyzed by electrophoresis on 2% agarose gels. .. RT-qPCR was performed with the ABI Real-time Polymerase Chain Reaction:Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions Article Snippet: Total RNA was extracted using RNeasy Plus Micro kit (Qiagen) according to the manufacturer’s protocol followed by cDNA synthesis using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. Real-time PCR was performed on an ABI Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions. Article Snippet: Objective: Dynorphin, an endogenous opioid peptide predominantly expressed in the central nervous system and involved in stress response, pain, and addiction, has intrigued researchers due to its expression in pancreatic b-cells.. In this study, we aimed to characterize dynorphin expression in mouse and human islets and explore the mechanisms regulating its expression.. Methods: We used primary mouse and human islets with unbiased published datasets to examine how glucose and other nutrients regulate dynorphin expression and secretion in islets. Article Title: Inhibitory Effect of TCF7L2 on Pancreatic β-Cell Dedifferentiation via ERK/MAPK Signaling Pathway in Diabetes Article Snippet: Total RNA was extracted from the cell lines using TRIzol Reagent (Thermo Fisher Scientific, Inc.) according to the manufacturer’s instructions. .. Reverse transcription was performed using a Reverse Transcription Kit (Thermo Fisher Scientific, Inc.). qPCR was performed in triplicate using the SYBR Green Master Mix (Takara Bio, Inc., Otsu, Japan) on an ABI Gene Expression:Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions Article Snippet: Total RNA was extracted using RNeasy Plus Micro kit (Qiagen) according to the manufacturer’s protocol followed by cDNA synthesis using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). .. Real-time PCR was performed on an ABI Article Title: Enhanced dynorphin expression and secretion in pancreatic beta-cells under hyperglycemic conditions. Article Snippet: Objective: Dynorphin, an endogenous opioid peptide predominantly expressed in the central nervous system and involved in stress response, pain, and addiction, has intrigued researchers due to its expression in pancreatic b-cells.. In this study, we aimed to characterize dynorphin expression in mouse and human islets and explore the mechanisms regulating its expression.. Methods: We used primary mouse and human islets with unbiased published datasets to examine how glucose and other nutrients regulate dynorphin expression and secretion in islets. Reverse Transcription:Article Title: Inhibitory Effect of TCF7L2 on Pancreatic β-Cell Dedifferentiation via ERK/MAPK Signaling Pathway in Diabetes Article Snippet: Total RNA was extracted from the cell lines using TRIzol Reagent (Thermo Fisher Scientific, Inc.) according to the manufacturer’s instructions. .. Reverse transcription was performed using a Reverse Transcription Kit (Thermo Fisher Scientific, Inc.). qPCR was performed in triplicate using the SYBR Green Master Mix (Takara Bio, Inc., Otsu, Japan) on an ABI Article Title: Serum amyloid P (PTX2) attenuates hepatic fibrosis in mice by inhibiting the activation of fibrocytes and HSCs Article Snippet: RNA was isolated from livers or isolated HSCs using TRIzol (Invitrogen) followed by an RNeasy kit (Qiagen). .. Quantitative reverse transcription polymerase chain reaction was performed using an ABI |